Adult The purpose of this procedure is to record the respiratory function of mice after sensitization with an inert allergen (ovalbumin; OVA) in response to challenge with a non-allergen airway agonist (Methacholine; MCh) using unrestrained whole-body plethysmography and assess the ability of the immune system to mount an acquired Type I allergic hypersensitivity immune response (allergic airway disease). 3.1 false Challenge Whole Body Plethysmography Unrestricted TCP_CHL_003